Effect of storage and disinfection methods of extracted bovine teeth on bond strength to dentin

Correspondence to: Marcelo Giannini Operative Dentistry Section Department of Restorative Dentistry Piracicaba School of Dentistry State University of Campinas Av. Limeira, 901 Piracicaba, SP, Brazil Zip code: 13418-903 Phone: 55 19 21065338 Fax: 55 19 21065218 E-mail: [email protected] Abstract The aim of this study was to evaluate the effect of storage and disinfection methods (SDM) on bond strength (BS) to bovine dentin, using two adhesive systems: Adper Single Bond and Clearfil Protect Bond. Method: Extracted bovine teeth were assigned to the following SDM: 100% Humidity (HU); Gamma Radiation (GR); Autoclave (AU); 0.10g/mL Thymol (TH); 10% Formalin (FO); Frozen (FR); 0.2% Sodium Azide (SA) and 0.5% Chloramine T (CT) (n=10). The GR and AU groups were submitted to sterilization methods and stored at 100% humidity for 24 hours at 37C°, before testing. TH, FO, FR, SA and CT groups were stored for three months at 5C°, except for FR (-4C°). The adhesive systems were applied according to manufacturer’s instructions. For BS testing, the micro-shear test was performed, using Tygon mold (0.75mm diameter and 1mm high), which was filled with Clearfil AP-X (Kuraray) composite resin. Data were analyzed by two-way ANOVA and Tukey Test (p<0.05). BS of adhesive systems was similar for all SDM, except for 10% formalin. HU, GR, AU, TH and SA did not change the BS for the adhesives tested. For most of SDM, BS of adhesive systems was similar. Some 3 month-SDM groups (FR, FO and CT) reduced the BS.